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The pea hsp70(LP19) promoter region, including 1.8 kb 5′ flanking sequence, and the first 18 amino acids of the coding region, was fused with coding sequence for P glucuronidase (GUS). Tobacco plants were transformed with this chimaeric gene in order to study tissue specific and developmental expression of the hsp70(LP19) promoter. Histological staining of GUS activity in transgenic tobacco plants showed that protein was present predominantly in the phloem tissue in stem, root and petioles In addition, developmental expression of the hsp70(LP19) gene promoter, without heat shock, was observed in petals, pollen grains, developing seeds as well as in germinating seeds and seedlings at different stages of growth.

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